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rfp trap magnetic agarose beads  (Proteintech)


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    Structured Review

    Proteintech rfp trap magnetic agarose beads
    Rfp Trap Magnetic Agarose Beads, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 79 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rfp+trap+magnetic+agarose+beads/NR4A3+Antibody/pm40336096-120-3-7
    Average 95 stars, based on 79 article reviews
    rfp trap magnetic agarose beads - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    other:

    Article Title: Studies on the Japanese soil-borne wheat mosaic virus movement protein highlight its ability to bind plant RNA
    Article Snippet: For the IPs, RFP-Trap magnetic agarose beads (Chromotek, Proteintech, Chicago, USA) were used according to the manufacturer’s protocol with minor changes.

    Article Title: Studies on the Japanese soil-borne wheat mosaic virus movement protein highlight its ability to bind plant RNA.
    Article Snippet: For the IPs, RFP-Trap magnetic agarose beads (Chromotek, Proteintech, Chicago, USA) were used according to the manufacturer’s protocol with minor changes.

    Incubation:

    Article Title: Regulation of Microprocessor assembly and localization via Pasha’s WW domain in C. elegans
    Article Snippet: .. 20 ul ChromoTek GFP-Trap Magnetic Agarose Beads (Proteintech, gtma-100) or ChromoTek RFP-Trap Magnetic Agarose Beads (Proteintech, rtma-20) were washed 3x in 500 ul wash buffer (25mM Tris HCL pH 7.5, 150 mM NaCl, 1.5 mM MgCl2, and 1 mM DTT) and then incubated with cleared cell lysates overnight while rotating at 4°C. ..

    Article Title: Regulation of Microprocessor assembly and localization via Pasha’s WW domain in C. elegans
    Article Snippet: .. For GFP and mCherry co-IPs, cell lysates were incubated with 25 ul ChromoTek GFP-Trap Magnetic Agarose Beads (Proteintech, gtma-100) (GFP co-IPs) or ChromoTek RFP-Trap Magnetic Agarose Beads (Proteintech, rtma-20) (mCherry co-IPs) for 1 hour at 4°C. .. For FLAG co-IPs, lysates were incubated with 7 ug FLAG antibody (Sigma, F3165) for 1 hour at 4°C.

    Article Title: FARL-11 (STRIP1/2) is required for sarcomere and sarcoplasmic reticulum organization in <i>C. elegans</i>
    Article Snippet: The supernatant was then precleared using ChromoTek control magnetic agarose beads (ChromoTek, cat. no. gmab20) for 30 min at 4°C. .. Precleared lysates were then incubated with ChromoTek RFP-Trap magnetic agarose beads (cat. no. rtma-20) for 1 h at 4°C to bind to TagRFP-myc-CASH-1. .. Beads were washed 3× with 1 ml of Lysis (−) buffer and eluted with 50 μl of Lysis (−) buffer at or IP were separated on a 10% SDS–PAGE, transferred to nitrocellulose membrane, and as shown in Figure 1D, one portion reacted against anti-C-Myc (catalogue number M4439, Sigma-Aldrich, St. Louis, MO at 1:3000 dilution), or affinity-purified anti-FARL-11 at 1:1000 dilution, reacted with the appropriate horseradish peroxidase (HRP)-conjugated secondary antibodies, and visualized with enhanced chemiluminescence (ECL) reagents (cat. no. 32106, Thermo Scientific) and exposure to film.

    Immunoprecipitation:




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